The importance of the research was to study the effectiveness of the cognitive technique and its relationship to the level of the enzyme acetylcholine and lactic acid and the most important offensive skills in volleyball for players, as the problem of the research is that the volleyball court is characterized by a relatively small size, and this necessitates the player to possess special qualities to perform the motor duty, therefore he made a decision. To get the greatest outcomes, the researchers looked into how the enzyme acetylcholine affected the efficacy of the cognitive approach, lactic acid, and offensive skills. The study's goals were to measure volleyball players' levels of cognitive technique efficacy, acetylcholinesterase enzyme activity, lactic acid production, and offensive skill levels. The researchers used the descriptive approach in the form of a survey and correlational studies on young Middle Euphrates volleyball club players competing in the premier league for the 2022-2023 athletic season to identify the correlation between the level of effectiveness of cognitive technology and the enzyme acetylcholinesterase and lactic acid for volleyball players. After excluding the players who hold the positions of prepared player and free player, the research sample, which consisted of 20 players from the Kufa Sports Club in the Al-Najaf Governorate and the Al-Daghara Sports Club in the Diwaniyah Governorate, was chosen at random. The tests that were used by the researchers included first determining the usefulness of cognitive technology, then determining the levels of lactic acid and the enzyme acetylcholine. Third: Evaluation of volleyball attacking performance. The researchers came to the conclusion that the psychological influence on the players raised the level of efficacy of their cognitive skill. Lactic acid content in the muscle causes the enzyme acetylcholinesterase to function less efficiently, which causes instructions to be sent more slowly. Increasing the amount of lactic acid in the muscles as a result of cognitive technology's greater efficacy. It was advised that volleyball coaches use the study's findings to understand the psychological and physical effects of their athletes. Due to its significance in reflecting on the physical and physiological aspect and consequently on performance, it is important to pay attention to the general psychological preparation of the players during the training process, along with the physical, skill, and tactical preparation.