The aim of the study was to find out the gender difference in Personality traits of Inter collegiate male and Female Kho Kho players with regard to psychoticism, neuroticism, extraversion, and Lie score. For this present study, 50 male and 50 female Kho Kho players were selected as a subject. The Esyenck Personality Inventory (E.P.I.) was used to measure Psychoticism, extraversion and neuroticism of Kho Kho players, tratios has been used to compare the significantly gender difference between male and female Kho Kho players who were participated in Inter collegiate Kho Kho tournament held at C.C.S. University, Meerut, Uttar Pradesh. Gender differences on Psychoticism was found between male and female Kho Kho players (t = 2.87; p < .05) where female players more psychotic than male. While analyzing the differences of Personality characteristic of male and female Kho Kho players, gender differences on neuroticism was found between male and female Inter collegiate Kho Kho players (t=3.52, p < .01), where the male Kho Kho players was found to have less score on neuroticism. So, far extraversion was concerned, significant gender difference was found to the male and female Inter- Collegiate Kho Kho players (t=2.56, p<.05), male Kho Kho players has lower extraversion. Hence, a female Kho Kho player was more extravert.