Methodology: For the purpose of this study 05 male National/ All India inter University sprinters were selected as subject. The age of the subject ranged from 18-25 years. The subject selected from Lucknow, Allahabad, and Varanasi District. The performance of the subject’s taking by filming protocol by using Casio ex-f1 high speed camera. Silicon Coach Pro-7 Motion analysis software was used for collecting raw data. The data was calculated in meter and hertz. The data for the selected variables were obtained with the help of filming protocol by using Casio ex-f1 high speed camera and further analyzed by using Silicon Coach Pro-7 Motion analysis software by an expert during the sprint performance. The data has been recorded only acceleration zone (50 meter distance) of the 100 meter race. There were two trials had given to the each subjects and the best trial was used for the analysis. Results of the study have shown that, mean value of Stride Length (4.2080) is higher than the stride rate (2.150). This means that the Stride Length plays important role in sprint Performance in acceleration zone. The acceleration phase is the most important phase in a race. During this phase, after the sprinter has left the blocks, the athlete increases the length of their stride and decreases the amount of strides taken per second.

Conclusion: Stride Length showed the higher affective variable which influence the Performance than Stride Rate. Acceleration Zone is the most important phase in the race.in this phase the athlete increases the length of their stride and decreases the amount of strides taken per second.